The Gut-on-chip Core Facility (GCF) aims to provide microfluidic technology to model intestinal environment for studying the environment-microbiota-host interactions. The GoC system is an organ-on-a-chip system based on microfluidic technology that enables the co-culture of host cells (epithelial, immune, pulmonary, organoid) and microbial cells (monoculture, mix or microbiota) under conditions representative of the physiological context. HuMiX consists of 3 microchannels for host cells and micro-organisms separated by two porous membranes, enabling exchanges between the compartments. Such system generates and maintains a physiologically relevant oxygen gradient (e.g., intestine, lung), representing a major asset in the study of interactions between the microbiota and the host. Samples (medium and cells) can be collected for functional analyses (omics, enzyme/cytokine assays, etc.).