Mathieu holds a PhD in microbiology, obtained in 2013, under the supervision of François Cornet, at the LMGM institute in Toulouse and worked on the terminal region of the Escherichia coli chromosome using fluorescent tags, for loci visualization, showing that a very specific dynamic of this region occurs in vivo.
Being interested in the global dynamic of the E.coli chromosome, Mathieu went to work in the laboratory of Nancy Kleckner at Harvard University, in Cambridge, USA, for a postdoctoral position. There, he studied the fluctuations of the nucleoid using high-throughput fluorescent microscopy to show that the nucleoid has a « breathing-like » cycle, and that these cycles of expansion/compaction depend on the level of superhelicity of the chromosome.
Since 2022, Mathieu is a research Engineer in the MuSE team working on the mutation rate in bacteriophages and E.coli, using the high-throughput methods developed in the lab for mutation visualization assays.
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